1GKY

REFINED STRUCTURE OF THE COMPLEX BETWEEN GUANYLATE KINASE AND ITS SUBSTRATE GMP AT 2.0 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	50.800, 50.800, 155.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 * - 2.000
R-factor	0.173 *
Rwork	0.173
RMSD bond length	0.016
RMSD bond angle	3.000
Refinement software	SIMULATED (ANNEALING METHOD)

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.050 *
Number of reflections	14130 *
Completeness [%]	100.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	GK	7 (mg/ml)
2	1	drop	potassium phosphate	40 (mM)
3	1	drop	GMP	1 (mM)
4	1	drop	PEG1500	1 (%(w/v))
5	1	drop	ammonium sulfate	1.0 (M)
6	1	reservoir	potassium phosphate	40 (mM)
7	1	reservoir	ammonium sulfate	2.0 (M)