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1GKQ

D-Hydantoinase (Dihydropyrimidinase) from Thermus sp. in space group P212121

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsENRAF-NONIUS FR591
Temperature [K]283
Detector technologyIMAGE PLATE
Collection date1997-11-06
DetectorMAC SCIENCE DIP-2030H
Spacegroup nameP 21 21 21
Unit cell lengths83.200, 161.500, 168.500
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution25.000 - 2.600
R-factor0.1876
Rwork0.188
R-free0.21100

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Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1gkp
RMSD bond length0.006
RMSD bond angle1.540

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Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareCNS
Refinement softwareCNS (1.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]25.0002.690
High resolution limit [Å]2.6002.600
Rmerge0.1170.363
Total number of observations416020

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Number of reflections70394
<I/σ(I)>124.9
Completeness [%]99.9

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99.9
Redundancy5.95.7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion, sitting drop

*

6.530% (W/V) PEG 6000, 100 MM CACODYLATE/HCL PH 6.5, 200 MM SODIUM ACETATE
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11reservoirPEG600026 (%)
21reservoircacodylate100 (mM)pH6.5
31reservoirsodium acetate200 (mM)
41dropprotein10 (mg/ml)

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