1GKI
Plasmid coupling protein TrwB in complex with ADP and Mg2+.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 151.100, 151.100, 251.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 3.000 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e9r |
RMSD bond length | 0.011 |
RMSD bond angle | 1.650 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.300 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.118 | 0.418 |
Number of reflections | 67169 | |
<I/σ(I)> | 5.9 | 1.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.4 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | pH 7.50 |