1GK4

HUMAN VIMENTIN COIL 2B FRAGMENT (CYS2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM1A
Synchrotron site	ESRF
Beamline	BM1A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-09-15
Spacegroup name	I 2 2 2
Unit cell lengths	76.594, 84.283, 240.785
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.520 - 2.300
R-factor	0.242
Rwork	0.242
R-free	0.26200
Structure solution method	MIRAS
RMSD bond length	0.008
RMSD bond angle	16.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.330
High resolution limit [Å]	2.300	2.300
Rmerge	0.058	0.405
Number of reflections	34937	1159 *
<I/σ(I)>	13.5	1.8
Completeness [%]	99.5	99.7
Redundancy	4.5	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	20 *	Strelkov, S.V., (2001) J.Mol.Biol., 306, 773 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-15 (mg/ml)