1GJY
The X-ray structure of the Sorcin Calcium Binding Domain (SCBD) provides insight into the phosphorylation and calcium dependent processess
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 130.925, 103.847, 78.546 |
Unit cell angles | 90.00, 117.96, 90.00 |
Refinement procedure
Resolution | 14.000 - 2.200 |
R-factor | 0.223 * |
Rwork | 0.232 |
R-free | 0.29600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 15.000 |
High resolution limit [Å] | 2.200 * |
Rmerge | 0.036 |
Number of reflections | 58335 |
Completeness [%] | 91.3 * |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.7 | 293 * | pH 5.70 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7 (mg/ml) | |
2 | 1 | reservoir | MES | 0.1 (M) | pH5.4-5.7 |
3 | 1 | reservoir | ammonium sulfate | 16 (%) |