1GJW

Thermotoga maritima maltosyltransferase complex with maltose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Spacegroup name	P 41 2 2
Unit cell lengths	147.390, 147.390, 105.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.100
R-factor	0.197
Rwork	0.197
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	FREE ENZYME
RMSD bond length	0.012
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.170
High resolution limit [Å]	2.100	2.130
Rmerge	0.044	0.247
Total number of observations	105717 *
Number of reflections	53029
<I/σ(I)>	17.9	3.7
Completeness [%]	81.0 *	56 *
Redundancy	2	1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.8	290 *	Burke, J., (2000) Acta Crystallog., D56, 1049. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	50 (mM)
2	1	drop		150 (mM)
3	1	drop	protein	18 (mg/ml)
4	1	reservoir	ammonium phosphate	0.35-0.40 (M)