1GJP
SCHIFF-BASE COMPLEX OF YEAST 5-AMINOLAEVULINIC ACID DEHYDRATASE WITH 4-OXOSEBACIC ACID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-15 |
| Detector | ADSC CCD |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 103.500, 103.500, 168.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.400 - 1.800 |
| R-factor | 0.265 |
| R-free | 0.32900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aw5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.020 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.400 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.059 | 0.425 |
| Number of reflections | 39906 | |
| <I/σ(I)> | 4.9 | 1.5 |
| Completeness [%] | 94.6 | 87.7 |
| Redundancy | 5.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 | Erskine, P.T., (1997) Protein Sci., 6, 1774. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 2 (mg/ml) | |
| 2 | 1 | reservoir | PEG6000 | 50 (%(w/v)) | |
| 3 | 1 | reservoir | zinc salfate | 4mg | |
| 4 | 1 | reservoir | beta-mercaptoethanol | 0.100ml | |
| 5 | 1 | reservoir | water | 0.010ml |
