1GJI
Crystal structure of c-Rel bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 168 |
| Detector technology | AREA DETECTOR |
| Collection date | 1999-02-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 99.320, 99.320, 196.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.850 |
| R-factor | 0.227 * |
| Rwork | 0.227 |
| R-free | 0.27900 |
| Structure solution method | MR and MIR |
| Starting model (for MR) | p65/DNA complex |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.320 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.086 | 0.424 |
| Total number of observations | 215187 * | |
| Number of reflections | 21455 * | |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 90.0 * | 48 * |
| Redundancy | 10 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 298 | PEG 4000, spermine, DTT, pH 7.5, EVAPORATION, temperature 298.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | spermine | ||
| 3 | 1 | 1 | DTT |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 20 (%(w/v)) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 4 | 1 | reservoir | spermine | 1 (mM) | |
| 5 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 6 | 1 | reservoir | beta-octylglucoside | 0.01 (%) |
