1GIT
STRUCTURE OF GTP-BINDING PROTEIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993 |
| Detector | FUJI |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.100, 77.100, 144.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.27900 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 22.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 * | |
| High resolution limit [Å] | 2.600 * | |
| Rmerge | 0.030 * | 0.090 * |
| Total number of observations | 28864 * | |
| Number of reflections | 11856 | |
| Completeness [%] | 84.1 | 86.5 * |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-10 (mg/ml) | |
| 2 | 1 | drop | 2.5 (mM) | ||
| 3 | 1 | drop | EDTA | 0.25 (mM) | |
| 4 | 1 | drop | dithiothreitol | 2.5 (mM) | |
| 5 | 1 | drop | GTPgammaS | 2.5 (mM) | |
| 6 | 1 | drop | NaEPPS | 12.5 (mM) | |
| 7 | 1 | drop | ammonium sulfate | 1.1-1.25 (M) | |
| 8 | 1 | reservoir | ammonium sulfate | 2.2-2.5 (M) |
