1GIQ
Crystal Structure of the Enzymatic Componet of Iota-Toxin from Clostridium Perfringens with NADH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 283 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.200, 54.440, 103.380 |
| Unit cell angles | 99.20, 93.10, 106.90 |
Refinement procedure
| Resolution | 20.000 * - 1.800 |
| Rwork | 0.224 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1GIR:THE ENZYMATIC COMPONENT IOTA-TOXIN WITH NADPH |
| RMSD bond length | 0.005 |
| RMSD bond angle | 22.700 * |
| Data reduction software | MOSFLM |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.256 |
| Number of reflections | 87691 | |
| <I/σ(I)> | 6 | |
| Completeness [%] | 95.9 | 93.4 |
| Redundancy | 1.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | 4 * | PEG4000, NADH, temperature 277.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris | 10 (mM) | pH7.5 |
| 3 | 1 | reservoir | PEG4000 | 18 (%) | |
| 4 | 1 | reservoir | NADH | 16 (mM) | |
| 5 | 1 | reservoir | Tris | 10 (mM) | pH7.5 |
