1GIK
POKEWEED ANTIVIRAL PROTEIN FROM SEEDS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-04-22 |
| Detector | FUJI |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 84.760, 78.050, 91.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.800 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.24200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1paf |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.698 * |
| Data reduction software | CCP4 |
| Data scaling software | WEIS |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.062 | 0.296 * |
| Total number of observations | 68617 * | |
| Number of reflections | 23204 * | |
| Completeness [%] | 82.0 * | 69.5 * |
| Redundancy | 3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.5 | 306 * | Li, H.M., (1998) ACTA CRYSTALLOGR.,SECT.D, 54, 137. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 100 (mg/ml) | |
| 2 | 1 | drop | 0.03 (N) | ||
| 3 | 1 | drop | PEG6000 | 30 (%(w/v)) | |
| 4 | 1 | drop | 0.2 (M) | pH5.0-6.0 | |
| 5 | 1 | reservoir | PEG6000 | 30 (%) |
