1GIH

HUMAN CYCLIN DEPENDENT KINASE 2 COMPLEXED WITH THE CDK4 INHIBITOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	294
Detector technology	IMAGE PLATE
Collection date	1998-12-07
Spacegroup name	P 21 21 21
Unit cell lengths	73.870, 72.670, 53.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.169 *
Rwork	0.169
R-free	0.27500
RMSD bond length	0.010
RMSD bond angle	1.677 *
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	AMoRE
Refinement software	X-PLOR (98.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.075	0.075
Total number of observations	38408 *
Number of reflections	7545
<I/σ(I)>	9
Completeness [%]	99.9	99.9
Redundancy	5.1	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.4 *	4 *	HEPES, sodium chloride, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 278K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES-NaOH	10 (mM)	pH7.4
3	1	drop		15 (mM)
4	1	reservoir	HEPES-NaOH	800 (mM)	pH7.4