1GHZ
A NOVEL SERINE PROTEASE INHIBITION MOTIF INVOLVING A MULTI-CENTERED SHORT HYDROGEN BONDING NETWORK AT THE ACTIVE SITE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1999-09-20 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 54.910, 54.910, 109.420 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 7.500 - 1.390 |
R-factor | 0.175 * |
Rwork | 0.175 |
R-free | 0.21200 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.017 |
RMSD bond angle | 3.700 |
Data reduction software | bioteX |
Data scaling software | bioteX |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.470 | 1.450 |
High resolution limit [Å] | 1.320 | 1.390 |
Rmerge | 0.089 | 0.285 |
Total number of observations | 74936 * | |
Number of reflections | 30747 | |
<I/σ(I)> | 7.7 | |
Completeness [%] | 67.1 | 31.3 |
Redundancy | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.1 | 298 | magnesium sulfate soak at pH 7.0. vapor diffusion at 298 K, pH 8.10 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | HEPES | 0.10 (M) | pH7.5 or 8.2 |
3 | 1 | reservoir | 0.30 (M) | ||
4 | 1 | reservoir | PEG5000 MME | 22 (%(v/v)) |