1GHV

A NOVEL SERINE PROTEASE INHIBITION MOTIF INVOLVING A MULTI-CENTERED SHORT HYDROGEN BONDING NETWORK AT THE ACTIVE SITE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2000-04-26
Detector	RIGAKU RAXIS IV
Spacegroup name	C 1 2 1
Unit cell lengths	71.620, 72.070, 73.200
Unit cell angles	90.00, 101.14, 90.00

Refinement procedure

Resolution	7.000 - 1.850
R-factor	0.18 *
Rwork	0.188
R-free	0.23500
RMSD bond length	0.019
RMSD bond angle	3.700
Data reduction software	SADIE
Data scaling software	SADIE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.180	1.870
High resolution limit [Å]	1.400	1.790
Rmerge	0.113	0.233
Total number of observations	41238 *
Number of reflections	25435
<I/σ(I)>	4.6
Completeness [%]	35.2	40.1
Redundancy	1.60 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8	298	PEG 4000, NaCl, pH 7.8, vapor diffusion, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	HEPES	0.10 (M)	pH7.5 or 8.2
3	1	reservoir		0.30 (M)
4	1	reservoir	PEG5000 MME	22 (%(v/v))