1GHQ
CR2-C3D COMPLEX STRUCTURE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 287 |
Spacegroup name | H 3 2 |
Unit cell lengths | 170.000, 170.000, 174.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 2.040 |
R-factor | 0.208 * |
Rwork | 0.195 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | |
High resolution limit [Å] | 2.040 * | |
Rmerge | 0.067 | 0.223 * |
Total number of observations | 65612 * | |
Number of reflections | 260000 | |
<I/σ(I)> | 10 | |
Completeness [%] | 94.1 * | 83.6 * |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.36 * | pH 6.0 | |
1 | Vapor diffusion, hanging drop * | 7.36 * | pH 6.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | PEG2000 | 17 (%) | |
3 | 1 | reservoir | zinc acetate | 0.2 (M) | |
4 | 1 | reservoir | sodium cacodylate | 0.1 (M) |