1GH2

Crystal structure of the catalytic domain of a new human thioredoxin-like protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	115
Detector technology	IMAGE PLATE
Collection date	2000-08-28
Detector	MAR scanner 345 mm plate
Spacegroup name	C 1 2 1
Unit cell lengths	87.500, 48.500, 29.800
Unit cell angles	90.00, 99.59, 90.00

Refinement procedure

Resolution	15.000 - 2.220
Rwork	0.221
R-free	0.25300
RMSD bond length	0.020 *
RMSD bond angle	1.970 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.300
High resolution limit [Å]	2.220	2.220
Rmerge	0.089	0.316
Number of reflections	6710 *	624 *
<I/σ(I)>	8.4
Completeness [%]	99.8 *	98.7
Redundancy	7.2	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5	291	ammonium sulfate, pH 5.0, EVAPORATION, temperature 291.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate