1GGZ
CRYSTAL STRUCTURE OF THE CALMODULIN-LIKE PROTEIN (HCLP) FROM HUMAN EPITHELIAL CELLS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 110 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 63.380, 93.630, 24.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.183 * |
| Rwork | 0.183 |
| R-free | 0.22100 |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.640 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.043 | 0.208 * |
| Number of reflections | 23940 | |
| <I/σ(I)> | 30.5 | |
| Completeness [%] | 97.7 * | 78.7 |
| Redundancy | 5.57 * | 2.58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 * | 277 | MPD, Sodium Acetate, Ethanol, CaCl2, pH 4.5, EVAPORATION, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | pH7.5 |
| 3 | 1 | drop | 2 (mM) | ||
| 4 | 1 | reservoir | MPD | 27 (%) | |
| 5 | 1 | reservoir | ethanol | 15 (%) | |
| 6 | 1 | reservoir | 50 (mM) | ||
| 7 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.5 |
