1GGY
HUMAN FACTOR XIII WITH YTTERBIUM BOUND IN THE ION SITE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.060, 72.390, 135.990 |
| Unit cell angles | 90.00, 106.09, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fie |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.078 * | |
| Number of reflections | 49005 | |
| Completeness [%] | 75.9 | 42 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.2 | pH 6.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 1,2-propanediol | 24 (%) | |
| 2 | 1 | 1 | sodium potassium phosphate | 100 (mM) |
