1GGQ
OUTER SURFACE PROTEIN C (OSPC) OF BORRELIA BURGDORFERI STRAIN B31
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-16 |
| Detector | BRANDEIS |
| Spacegroup name | P 1 |
| Unit cell lengths | 127.206, 33.654, 47.993 |
| Unit cell angles | 84.04, 81.55, 89.23 |
Refinement procedure
| Resolution | 40.740 - 2.510 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.27000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.130 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.043 | 0.142 |
| Number of reflections | 24505 * | |
| <I/σ(I)> | 16.1 | |
| Completeness [%] | 91.1 * | 75.4 |
| Redundancy | 3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8.5 | 4 * | 23 % (w/v) PEG 3350, 50 mM Tris/HCl pH 8.5, 100 mM MgCl2, 10% (v/v) glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG3350 | 28-30 (%) | |
| 2 | 1 | 1 | Tris-HCl | 80 (mM) | |
| 3 | 1 | 1 | 100 (mM) | ||
| 4 | 1 | 1 | glycerol | 18 (%) |
