1GGM
GLYCYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS COMPLEXED WITH GLYCYL-ADENYLATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 272 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04-23 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 122.500, 250.100, 106.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.000 - 3.400 |
| Rwork | 0.242 |
| R-free | 0.33400 |
| Structure solution method | OTHER |
| RMSD bond length | 0.014 |
| RMSD bond angle | 25.300 * |
| Data reduction software | XDS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 3.400 |
| Rmerge | 0.073 |
| Number of reflections | 20549 |
| Completeness [%] | 91.1 |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 24 * | pH 8.0, temperature 297K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ATP | ||
| 2 | 1 | 1 | GLY | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | (NH4)2SO4 | ||
| 5 | 1 | 1 | TRIS HCL | ||
| 6 | 1 | 2 | (NH4)2SO4 | ||
| 7 | 1 | 2 | TRIS HCL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | GlyRS | 0.02 (mM) | |
| 2 | 1 | drop | ATP | 5 (mM) | or 50 mM ethanolamine |
| 3 | 1 | drop | 2 (mM) | ||
| 4 | 1 | drop | ammonium sulfate | 0.4 (M) | |
| 5 | 1 | drop | Tris-HCl | 50 (mM) | |
| 6 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 7 | 1 | reservoir | ammonium sulfate | 1.0-1.4 (M) |
