1GG8

DESIGN OF INHIBITORS OF GLYCOGEN PHOSPHORYLASE: A STUDY OF ALPHA-AND BETA-C-GLUCOSIDES AND 1-THIO-BETA-D-GLUCOSE COMPOUNDS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1993-08-09
Detector	NICOLET
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	128.500, 128.500, 116.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.500 - 2.310
R-factor	0.202
Rwork	0.202
R-free	0.25300
Structure solution method	OTHER
Starting model (for MR)	2prj
RMSD bond length	0.007
RMSD bond angle	25.400 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CCP4
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	18.500	2.420
High resolution limit [Å]	2.310	2.310
Rmerge	0.092
Total number of observations	85299 *
Number of reflections	34397
<I/σ(I)>	10.8
Completeness [%]	79.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	6.7		Oikonomakos, N.G., (1985) Biochim. Biophys. Acta, 832, 248. *