1GG4
CRYSTAL STRUCTURE OF ESCHERICHIA COLI UDPMURNAC-TRIPEPTIDE D-ALANYL-D-ALANINE-ADDING ENZYME (MURF) AT 2.3 ANGSTROM RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-08-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 |
| Unit cell lengths | 74.200, 74.200, 429.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| Rwork | 0.203 |
| R-free | 0.28200 * |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.860 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.065 | 0.338 |
| Total number of observations | 545211 * | |
| Number of reflections | 47573 * | |
| Completeness [%] | 82.0 | 54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 * | 4 * | PEG 8000, magnesium sulfate, glycerol, pH 9.4, EVAPORATION, temperature 278K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 13 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | dithiothreitol | 10 (mM) | |
| 4 | 1 | reservoir | PEG8000 | 16 (%(w/v)) | |
| 5 | 1 | reservoir | bis-Tris | 0.1 (M) | |
| 6 | 1 | reservoir | 0.12 (M) | ||
| 7 | 1 | reservoir | glycerol | 10 (%(w/v)) |
