1GFI

STRUCTURES OF ACTIVE CONFORMATIONS OF GI ALPHA 1 AND THE MECHANISM OF GTP HYDROLYSIS

Experimental procedure

Temperature [K]	100
Spacegroup name	P 32 2 1
Unit cell lengths	80.500, 80.500, 106.550
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.214
Rwork	0.214
R-free	0.28400
RMSD bond length	0.012 *
RMSD bond angle	1.500 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.200 *
Rmerge	0.053 *
Total number of observations	96580 *
Number of reflections	19715
Completeness [%]	97.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6 *	21 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25-30 (mg/ml)
2	1	drop		0.03 (M)
3	1	drop		5 (mM)
4	1	drop		5 (mM)
5	1	drop	dithiothreitol	10 (mM)
6	1	drop	sodium acetate	200 (mM)
7	1	reservoir	ammonium sulfate	1.8-1.9 (M)