1GEX
CRYSTAL STRUCTURE OF HISTIDINOL-PHOSPHATE AMINOTRANSFERASE COMPLEXED WITH HISTIDINOL-PHOSPHATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-12-25 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.924, 63.836, 46.365 |
| Unit cell angles | 90.00, 104.06, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.195 |
| Rwork | 0.191 |
| R-free | 0.26100 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.411 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.086 | 0.231 |
| Total number of observations | 87507 * | |
| Number of reflections | 18942 | |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 98.4 | 95.5 |
| Redundancy | 3.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.7 | 293 | PEG 4000, magnesium chloride, tris, pH 7.7, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20-25 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 17.5 (%(w/v)) | |
| 3 | 1 | reservoir | Hsp | 82 (mm) | |
| 4 | 1 | reservoir | 200 (mM) | ||
| 5 | 1 | reservoir | Tris-HCl | 100 (mM) |
