1GEN

C-TERMINAL DOMAIN OF GELATINASE A

Experimental procedure

Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS
Spacegroup name	P 21 21 21
Unit cell lengths	57.900, 77.400, 53.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.500 - 2.150
R-factor	0.188
Rwork	0.188
R-free	0.26000
RMSD bond length	0.016
RMSD bond angle	25.700 *
Data reduction software	R-AXIS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.000
Rmerge	0.030
Number of reflections	13690
Completeness [%]	92.0
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	gA-Ctd	10 (mg/ml)
2	1	reservoir	PEG8000	20 (%)
3	1	reservoir	zinc acetate	0.15 (M)
4	1	reservoir	cacodylate	0.1 (M)