1GEK
STRUCTURAL CHARACTERIZATION OF N-BUTYL-ISOCYANIDE COMPLEXES OF CYTOCHROMES P450NOR AND P450CAM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-02-20 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.7 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 106.340, 102.740, 36.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.700 |
Rwork | 0.225 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cpp |
RMSD bond length | 0.011 * |
RMSD bond angle | 24.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.059 | 0.358 |
Number of reflections | 40825 | |
<I/σ(I)> | 7.6 | 2.3 |
Completeness [%] | 91.7 | 67.6 |
Redundancy | 7 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 7.4 * | 5 * | Imai, M., (1989) Proc. Natl. Acad. Sci., 86, 7823. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | enzyme | 0.008 (mM) | |
2 | 1 | 1 | potassium phosphate | 50 (mM) | |
3 | 1 | 1 | 50 (mM) | ||
4 | 1 | 1 | d-camphor | 1 (mM) |