1GEE
Crystal structure of glucose dehydrogenase mutant Q252L complexed with NAD+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 288 |
Detector technology | DIFFRACTOMETER |
Collection date | 1999-11-28 |
Detector | WEISSENBERG |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 120.528, 66.601, 119.296 |
Unit cell angles | 90.00, 93.14, 90.00 |
Refinement procedure
Resolution | 39.710 - 1.600 |
Rwork | 0.195 |
R-free | 0.20700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gco |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.063 | 0.415 |
Number of reflections | 318004 | |
<I/σ(I)> | 23.2 | |
Completeness [%] | 86.5 | 73.8 |
Redundancy | 2.95 | 2.83 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | sodium phosphate, PEG2000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |