1GDV

CRYSTAL STRUCTURE OF CYTOCHROME C6 FROM RED ALGA PORPHYRA YEZOENSIS AT 1.57 A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	293
Detector technology	CCD
Collection date	2000-06-20
Detector	MAR CCD 165 mm
Spacegroup name	P 43 21 2
Unit cell lengths	49.260, 49.260, 83.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.570
Rwork	0.199
R-free	0.25400
RMSD bond length	0.015
RMSD bond angle	25.800 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.650
High resolution limit [Å]	1.570	1.570
Rmerge	0.047	0.347
Total number of observations	105068 *
Number of reflections	14083
<I/σ(I)>	9.2
Completeness [%]	96.7	95
Redundancy	7.5	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7 *	293	1.8M ammonium sulfate, 0.1M sodium citrate, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	50 (mg/ml)
2	1	drop	sodium phosphate	5 (mM)
3	1	reservoir	ammonium sulfate	1.8 (M)
4	1	reservoir	sodium citrate	0.1 (M)