1GDV
CRYSTAL STRUCTURE OF CYTOCHROME C6 FROM RED ALGA PORPHYRA YEZOENSIS AT 1.57 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2000-06-20 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 49.260, 49.260, 83.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.570 |
| Rwork | 0.199 |
| R-free | 0.25400 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 25.800 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.650 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.047 | 0.347 |
| Total number of observations | 105068 * | |
| Number of reflections | 14083 | |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 96.7 | 95 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 293 | 1.8M ammonium sulfate, 0.1M sodium citrate, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 50 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 5 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
| 4 | 1 | reservoir | sodium citrate | 0.1 (M) |
