1GDU
FUSARIUM OXYSPORUM TRYPSIN AT ATOMIC RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 283 |
Detector technology | AREA DETECTOR |
Collection date | 1993-12-05 |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 33.290, 37.050, 40.259 |
Unit cell angles | 102.46, 103.81, 102.07 |
Refinement procedure
Resolution | 15.000 - 1.070 |
R-factor | 0.1036 |
Rwork | 0.104 |
RMSD bond length | 0.015 |
RMSD bond angle | 0.031 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.090 |
High resolution limit [Å] | 1.070 | 1.070 |
Rmerge | 0.083 | 0.083 |
Total number of observations | 297606 * | |
Number of reflections | 70387 * | |
<I/σ(I)> | 6.8 | |
Completeness [%] | 92.8 | 87.6 |
Redundancy | 4.2 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | sodium sulphate, sodium citrate, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 19K |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | sodium sulphate, sodium citrate, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 19K |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | sodium sulphate, sodium citrate, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 19K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | boric acid | 100 (mM) | |
2 | 1 | drop | 2 (mM) | ||
3 | 1 | drop | 100 (mM) | ||
4 | 1 | drop | 3,3-dimethyl glutaric acid | 10 (mM) | |
5 | 1 | drop | protein | 25 (mg/ml) | |
6 | 1 | reservoir | 1.4 (M) | ||
7 | 1 | reservoir | sodium citrate | 0.1 (M) | |
8 | 1 | reservoir | 2-propanol | 0-5 (%) |