1GDT
CRYSTAL STRUCTURE OF A SITE-SPECIFIC RECOMBINASE, GAMMA-DELTA RESOLVASE COMPLEXED WITH A 34 BP CLEAVAGE SITE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 113 |
| Collection date | 1994-04-01 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 113.000, 157.000, 37.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.000 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 33.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.000 |
| Rmerge | 0.077 |
| Number of reflections | 13255 |
| Completeness [%] | 94.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | resolvase monomer | 0.36 (mM) | |
| 10 | 1 | reservoir | ethylene glycol | 5 (%) | |
| 11 | 1 | reservoir | MES | 100 (mM) | |
| 12 | 1 | reservoir | PRG3350 | 30 (%) | |
| 2 | 1 | drop | DNA modular duplex | 0.72 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 0.2 (M) | |
| 4 | 1 | drop | ethylene glycol | 2.5 (%) | |
| 5 | 1 | drop | EDTA | 0.5 (mM) | |
| 6 | 1 | drop | Tris-HCl | 10 (mM) | |
| 7 | 1 | drop | MES | 50 (mM) | |
| 8 | 1 | drop | PEG3350 | 15 (%) | |
| 9 | 1 | reservoir | ammonium sulfate | 0.2 (M) |
