1GDH

CRYSTAL STRUCTURE OF A NAD-DEPENDENT D-GLYCERATE DEHYDROGENASE AT 2.4 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 1
Unit cell lengths	60.410, 60.530, 66.290
Unit cell angles	102.30, 113.73, 102.73

Refinement procedure

Resolution	10.000 * - 2.400
R-factor	0.194 *
Rwork	0.189
RMSD bond length	0.010
RMSD bond angle	1.570 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.400 *
Rmerge	0.048 *
Total number of observations	41961 *
Number of reflections	24903 *
Completeness [%]	80.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	18 *	Goldberg, J.D., (1992) J.Mol.Biol., 225, 909. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	potassium phosphate	10 (mM)
2	1	drop	dithiothreitol	0.1 (mM)
3	1	drop	protein	18 (mg/ml)
4	1	drop	ammonium sulfate	45 (%sat)
5	1	reservoir	ammonium sulfate	45 (%sat)