1GDD
TERTIARY AND QUATERNARY STRUCTURAL CHANGES IN GIA1 INDUCED BY GTP HYDROLYSIS
Experimental procedure
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-04-12 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | I 4 |
| Unit cell lengths | 121.840, 121.840, 68.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.200 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.29100 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 23.120 * |
| Data reduction software | DENZO |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 2.200 | 2.200 * |
| Rmerge | 0.052 | 0.412 * |
| Number of reflections | 23024 | |
| Completeness [%] | 95.0 | 70.6 * |
| Redundancy | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | activated Gialpha1 | 8 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 1.4 (M) | |
| 3 | 1 | drop | EPPS | 25 (mM) | |
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | drop | GTPgammaS | 0.5 (mM) | |
| 6 | 1 | drop | dithiothreitol | 2.5 (mM) | |
| 7 | 1 | drop | EDTA | 10 (mM) | |
| 8 | 1 | reservoir | 100 (mM) | ||
| 9 | 1 | reservoir | potassium phosphate | 1.45-1.65 (M) |
