1GD7
CRYSTAL STRUCTURE OF A BIFUNCTIONAL PROTEIN (CSAA) WITH EXPORT-RELATED CHAPERONE AND TRNA-BINDING ACTIVITIES.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-06-20 |
Detector | MAR CCD 165 mm |
Spacegroup name | P 43 |
Unit cell lengths | 55.715, 55.715, 167.064 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
R-factor | 0.217 * |
Rwork | 0.217 |
R-free | 0.27700 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.043 | 0.191 |
Total number of observations | 135225 * | |
Number of reflections | 33368 | |
<I/σ(I)> | 14.7 | |
Completeness [%] | 97.5 | 94.5 * |
Redundancy | 4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 20 * | NaCl, Sodium Acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 14 (mg/ml) | |
2 | 1 | reservoir | 3.8 (M) | ||
3 | 1 | reservoir | sodium acetate | 0.1 (M) |