1GD6

STRUCTURE OF THE BOMBYX MORI LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	283
Detector technology	IMAGE PLATE
Collection date	1999-08-09
Detector	MAC Science DIP-2000
Spacegroup name	P 41 21 2
Unit cell lengths	77.268, 77.268, 72.925
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.500
Rwork	0.181
R-free	0.22300 *
Structure solution method	MR
RMSD bond length	0.009
RMSD bond angle	1.550 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.080	0.300 *
Total number of observations	97639 *
Number of reflections	7862
<I/σ(I)>	20.9	3.8388
Completeness [%]	98.6	96.4
Redundancy	7.12	5.08 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	18 *	PEG 8000, ammonium sulfate, sodium cacodylate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	ammonium sulfate	0.2 (M)
3	1	drop	PEG8000	26 (%(w/v))
4	1	reservoir	sodium cacodylate	0.1 (M)