1GD0
HUMAN MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6B |
Synchrotron site | Photon Factory |
Beamline | BL-6B |
Temperature [K] | 100 |
Detector technology | DIFFRACTOMETER |
Collection date | 1998-10-18 |
Detector | WEISSENBERG |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.500, 67.500, 88.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.000 - 1.500 |
R-factor | 0.191 |
Rwork | 0.191 |
R-free | 0.21400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mif |
RMSD bond length | 0.013 |
RMSD bond angle | 26.100 * |
Data reduction software | DENZO |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | X-PLOR |
Refinement software | X-PLOR (98.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.540 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.026 | 0.359 |
Number of reflections | 56679 | |
<I/σ(I)> | 18.7 | |
Completeness [%] | 86.9 | 67.1 |
Redundancy | 2.7 | 1.95 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | ammonium sulfate, sodium citrate, pH 5.0, vapor diffusion : hanging drop, temperature 298.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 100 (mg/ml) | |
2 | 1 | drop | beta-mercaptoethanol | 14.4 (mM) | |
3 | 1 | drop | PMSF | 0.1 (mM) | |
4 | 1 | drop | sodium citrate | 100 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 0.6-1.5 (M) | |
6 | 1 | reservoir | HEPES | 0.1 (M) |