1GCV
DEOXY FORM HEMOGLOBIN FROM MUSTELUS GRISEUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 1999-11-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.680, 90.550, 61.450 |
| Unit cell angles | 90.00, 98.45, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.181 * |
| Rwork | 0.181 |
| R-free | 0.22300 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.146 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.044 | 0.166 |
| Total number of observations | 181593 * | |
| Number of reflections | 41763 * | |
| Completeness [%] | 98.8 | 98.1 |
| Redundancy | 4.3 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | 298 | ammonium sulfate, ammonium phosphate, hydro sulfide, pH 6.5, SMALL TUBES, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium sulfate | 1.7 (M) | |
| 2 | 1 | 1 | ammonium phosphate | 400 (mM) | |
| 3 | 1 | 1 | protein | 2.1 (%) |
