1GCU
CRYSTAL STRUCTURE OF RAT BILIVERDIN REDUCTASE AT 1.4 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-03-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.682, 70.487, 87.866 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.700 - 1.400 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.24800 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.710 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.079 | 0.324 |
| Total number of observations | 379020 * | |
| Number of reflections | 71182 * | |
| <I/σ(I)> | 6 | |
| Completeness [%] | 94.3 | |
| Redundancy | 5.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 283 | (NH4)2SO4, Na citrate, Na/K tartrate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium citrate | 0.1 (M) | |
| 2 | 1 | reservoir | potassium sodium tartrate | 0.2 (M) | |
| 3 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 4 | 1 | drop | protein | 4.3 (mg/ml) |
