1GCT

IS GAMMA-CHYMOTRYPSIN A TETRAPEPTIDE ACYL-ENZYME ADDUCT OF GAMMA-CHYMOTRYPSIN?

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	285
Detector technology	FILM
Detector	KODAK
Spacegroup name	P 42 21 2
Unit cell lengths	69.800, 69.800, 98.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 1.600
R-factor	0.173 *
Rwork	0.173
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	3.100
Data reduction software	OSCTST
Data scaling software	AGROVATA/ROTAVATE
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	1.600 *
Rmerge	0.066 *
Total number of observations	58476 *
Number of reflections	23255 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.5 *		Cohen, G.H., (1981) J. Mol. Biol., 148, 449. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	12.5 (mg/ml)
2	1	1	ammonium sulfate	50 (%sat)