1GCO
CRYSTAL STRUCTURE OF GLUCOSE DEHYDROGENASE COMPLEXED WITH NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 290 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1999-06-18 |
| Detector | WEISSENBERG |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.675, 66.557, 119.528 |
| Unit cell angles | 90.00, 93.21, 90.00 |
Refinement procedure
| Resolution | 34.390 - 1.700 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.19200 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.033 | 0.166 |
| Total number of observations | 357354 * | |
| Number of reflections | 94821 * | |
| <I/σ(I)> | 29.2 | |
| Completeness [%] | 91.9 | 77.3 |
| Redundancy | 3.77 | 3.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 * | used microseeding, Yamamoto, K., (2000) Acta Crystallogr., Sect.D, 56, 1443. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 40 (mM) | |
| 3 | 1 | reservoir | PEG2000 | 28 (%(w/v)) | |
| 4 | 1 | reservoir | NAD+ | 4 (mM) | |
| 5 | 1 | reservoir | sodium phosphate | 40 (mM) |
