1GCI
THE 0.78 ANGSTROMS STRUCTURE OF A SERINE PROTEASE-BACILLUS LENTUS SUBTILISIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-10 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.650, 61.250, 74.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 - 0.780 |
| R-factor | 0.0993 |
| R-free | 0.10260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jea |
| RMSD bond length | 0.012 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 0.820 |
| High resolution limit [Å] | 0.780 | 0.780 |
| Rmerge | 0.036 | 0.290 |
| Total number of observations | 795681 * | |
| Number of reflections | 257583 | |
| <I/σ(I)> | 5.8 | 2.6 |
| Completeness [%] | 97.3 | 92.7 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.9 | 37 * | FREE TEXT GOES HERE., pH 5.9 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 50 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | inhibitor | ||
| 5 | 1 | reservoir | ammonium sulfate | 30-33 (%sat) |
