1GC8
THE CRYSTAL STRUCTURE OF THERMUS THERMOPHILUS 3-ISOPROPYLMALATE DEHYDROGENASE MUTATED AT 172TH FROM ALA TO PHE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1999-10-13 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.255, 87.830, 71.186 |
Unit cell angles | 90.00, 100.02, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.500 |
R-factor | 0.17 |
Rwork | 0.170 |
R-free | 0.25800 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.334 * |
Phasing software | X-PLOR |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.750 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.056 | 0.184 |
Number of reflections | 20952 | |
Completeness [%] | 92.8 * | |
Redundancy | 3.15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 298 | Sakurai, M., (1992) J.Biochem., 112, 173. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 20 (mM) | pH7.6 |
3 | 1 | reservoir | ammonium sulfate | 1.0-2.0 (M) |