1GBV

(ALPHA-OXY, BETA-(C112G)DEOXY) T-STATE HUMAN HEMOGLOBIN

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1993-10-10
Detector	SIEMENS
Spacegroup name	P 1 21 1
Unit cell lengths	63.180, 83.560, 53.730
Unit cell angles	90.00, 99.60, 90.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.18 *
Rwork	0.184
RMSD bond length	0.020
RMSD bond angle	0.040 *
Data reduction software	XENGEN
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	6.000
High resolution limit [Å]	2.000
Rmerge	0.060
Total number of observations	50688 *
Number of reflections	34600
<I/σ(I)>	2.3
Completeness [%]	92.8 *
Redundancy	1.46

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5 *		Perutz, M.F., (1968) J. Crystal Growth, 2, 54. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	4 (M)
2	1	1		2 (M)
3	1	1		2 (M)