1GBT
STRUCTURE OF AN ACYL-ENZYME INTERMEDIATE DURING CATALYSIS: (GUANIDINOBENZOYL) TRYPSIN
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.740, 63.540, 68.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.000 |
| R-factor | 0.162 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 3.600 |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Rmerge | 0.092 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.15 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | trypsin | 60 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 0.05 (M) | |
| 3 | 1 | drop | 0.3 (M) | ||
| 4 | 1 | drop | 0.006 (M) | ||
| 5 | 1 | drop | benzamidine | 0.06 (M) | |
| 6 | 1 | drop | enzyme | 1.3 (mM) | |
| 7 | 1 | reservoir | Tris-HCl | 0.05 (M) | |
| 8 | 1 | reservoir | 1.6-1.8 (M) |
