1GBN

HUMAN ORNITHINE AMINOTRANSFERASE COMPLEXED WITH THE NEUROTOXIN GABACULINE

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	110
Detector technology	CCD
Collection date	1995-10-10
Detector	PRINCETON 2K
Spacegroup name	P 32 2 1
Unit cell lengths	115.013, 115.013, 185.753
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.206 *
Rwork	0.209
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1oat
RMSD bond length	0.007
RMSD bond angle	1.320 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.064	0.171 *
Number of reflections	57010
<I/σ(I)>	16.2	5.9
Completeness [%]	89.9 *	80.1
Redundancy	4.6	2.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8 *		pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop	PLP	0.01 (M)
4	1	reservoir	PEG6000	10-15 (%)
5	1	reservoir		50-200 (mM)