1GBA
ALPHA-LYTIC PROTEASE WITH MET 190 REPLACED BY ALA AND GLY 216 REPLACED BY ALA
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1994-01-27 |
| Detector | RIGAKU |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 66.180, 66.180, 80.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 17.500 - 2.150 |
| R-factor | 0.138 |
| Rwork | 0.138 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 25.800 * |
| Data reduction software | RIGAKU (AFC5 CONTROL SOFTWARE) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 17.500 |
| High resolution limit [Å] | 2.150 |
| Number of reflections | 10527 |
| Completeness [%] | 87.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 1.4 (M) | ||
| 2 | 1 | reservoir | Tris-HCl | 20 (mM) |
