1GAV
BACTERIOPHAGE GA PROTEIN CAPSID
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 280 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-08-24 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 272.700, 293.500, 339.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 3.400 |
| R-factor | 0.241 * |
| Rwork | 0.279 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 * |
| RMSD bond angle | 1.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.139 | 0.392 |
| Number of reflections | 126364 | 15134 * |
| <I/σ(I)> | 4.6 | 1.8 |
| Completeness [%] | 68.0 | 56 |
| Redundancy | 2.3 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | ammonium sulfate | 5 (%) | |
| 2 | 1 | drop | Tris-HCl | 0.04 (M) | |
| 3 | 1 | drop | 0.15 (M) | ||
| 4 | 1 | drop | 0.02 (%) | ||
| 5 | 1 | reservoir | 0.9 (M) | ||
| 6 | 1 | reservoir | Tris-HCl | 0.04 (M) |
