1GAV

BACTERIOPHAGE GA PROTEIN CAPSID

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	280
Detector technology	IMAGE PLATE
Collection date	1995-08-24
Detector	MARRESEARCH
Spacegroup name	I 2 2 2
Unit cell lengths	272.700, 293.500, 339.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 * - 3.400
R-factor	0.241 *
Rwork	0.279
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016 *
RMSD bond angle	1.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.580
High resolution limit [Å]	3.400	3.400
Rmerge	0.139	0.392
Number of reflections	126364	15134 *
<I/σ(I)>	4.6	1.8
Completeness [%]	68.0	56
Redundancy	2.3	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8		pH 8.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	5 (%)
2	1	drop	Tris-HCl	0.04 (M)
3	1	drop		0.15 (M)
4	1	drop		0.02 (%)
5	1	reservoir		0.9 (M)
6	1	reservoir	Tris-HCl	0.04 (M)