1GAN
COMPLEX OF TOAD OVARY GALECTIN WITH N-ACETYLGALACTOSE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-12 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 54.330, 54.330, 186.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.230 |
| R-factor | 0.1831 |
| Rwork | 0.183 |
| R-free | 0.24500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1slt |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.500 * |
| Data reduction software | R-AXIS (V. 3.4) |
| Data scaling software | R-AXIS (V. 3.4) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.500 |
| High resolution limit [Å] | 2.230 | 2.250 |
| Rmerge | 0.075 | 0.190 * |
| Number of reflections | 11873 * | |
| <I/σ(I)> | 10000 | 3.3 |
| Completeness [%] | 901.0 * | 73.1 |
| Redundancy | 7.5 | 7.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.6 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-12 (mg/ml) | |
| 2 | 1 | drop | LacNAc | 5 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 56 (%sat) | |
| 4 | 1 | reservoir | Tris-acetate | 100 (mM) | pH6.6 |
| 5 | 1 | reservoir | 2-methyl-2,4-pentanediol | 1 (%) | |
| 6 | 1 | reservoir | dithiothreitol | 1 (mM) |
