1GAI
GLUCOAMYLASE-471 COMPLEXED WITH D-GLUCO-DIHYDROACARBOSE
Experimental procedure
Source type | ROTATING ANODE |
Source details | SIEMENS |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Collection date | 1993-10-20 |
Detector | SIEMENS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 116.900, 104.400, 48.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.700 |
R-factor | 0.147 |
Rwork | 0.147 |
R-free | 0.16700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | NATIVE GLOCOAMYLASE |
RMSD bond length | 0.008 |
RMSD bond angle | 21.600 * |
Data reduction software | XENGEN |
Data scaling software | XENGEN |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.800 |
High resolution limit [Å] | 1.690 | 1.700 * |
Rmerge | 0.055 | 0.257 |
Total number of observations | 298712 * | |
Number of reflections | 64713 * | |
<I/σ(I)> | 36.16 | 3.3 |
Completeness [%] | 96.0 | 75 * |
Redundancy | 4.66 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 * | Golubev, A. M., (1992) J. Mol. Biol., 226, 271. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-15 (mg/ml) | |
2 | 1 | drop | PEG6000 | 30 (%(w/v)) | |
3 | 1 | drop | sodium phosphate | 0.15 (M) | |
4 | 1 | reservoir | PEG6000 | 30 (%(w/v)) | |
5 | 1 | reservoir | sodium phosphate | 0.15 (M) |