1GAF
48G7 HYBRIDOMA LINE FAB COMPLEXED WITH HAPTEN 5-(PARA-NITROPHENYL PHOSPHONATE)-PENTANOIC ACID
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-06-06 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.260, 44.850, 85.450 |
| Unit cell angles | 90.00, 121.55, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.244 |
| Rwork | 0.244 |
| R-free | 0.30760 * |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.613 |
| Data reduction software | DENZO |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.950 |
| Rmerge | 0.072 |
| Total number of observations | 121262 * |
| Number of reflections | 33282 |
| Completeness [%] | 96.7 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | 48G7 Fab | 15 (mg/ml) | |
| 2 | 1 | 1 | hapten | 1 (mM) | |
| 3 | 1 | 1 | sodium acetate | 100 (mM) | |
| 4 | 1 | 1 | sodium cacodylate | 100 (mM) | |
| 5 | 1 | 1 | PEG3300 | 27 (%) |
