1GA8
CRYSTAL STRUCTURE OF GALACOSYLTRANSFERASE LGTC IN COMPLEX WITH DONOR AND ACCEPTOR SUGAR ANALOGS.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-06-10 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.975, 76.158, 87.228 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.000 |
| R-factor | 0.193 * |
| Rwork | 0.189 |
| R-free | 0.22760 * |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1g9r |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.330 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.062 | 0.146 |
| Number of reflections | 18508 | |
| <I/σ(I)> | 21 | 8.1 |
| Completeness [%] | 98.2 | 89.6 |
| Redundancy | 8.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 291 | used seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | TCEP | 3 (mM) | |
| 2 | 1 | drop | 3 (mM) | ||
| 3 | 1 | drop | UDP-2FGal | 5 (mM) | |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | drop | 50 (mM) | ||
| 6 | 1 | reservoir | 50 (mM) | ||
| 7 | 1 | reservoir | PEG2000 MME | 5-20 (%(w/v)) |
